Binding of 1-Decylpyridinium Bromide to Poly(vinyl sulfate)
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Intra-articular Injection of HB-IGF-1 Sustains Delivery of IGF-1 to Cartilage through Binding to Chondroitin Sulfate
Objective—IGF-1 stimulates cartilage repair but is not a practical therapy due to its short halflife. We have previously modified IGF-1 by adding a heparin-binding domain and have shown that this fusion protein (HB-IGF-1) stimulates sustained proteoglycan synthesis in cartilage. Here, we first examined the mechanism by which HB-IGF-1 is retained in cartilage. We then tested whether HB-IGF-1 pro...
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In the title compound, C(19)H(34)N(+)·Br(-)·H(2)O, the dihedral angle between the trans-planar alkyl side chain and the pyridinium ring is 52.73 (7)°. In the crystal structure, O-H⋯Br, C-H⋯Br and C-H⋯O hydrogen bonds form a network, while the hydro-phobic alkyl chains inter-digitate, forming bilayers.
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Binding studies of cationic detergents such as cetyltrimethyl ammonium bromide, Cetyl pyridinium bromide and dodecyl trimethyl ammonium bromide with β Lactoglobulin were carried out by equilibrium dialysis, ultraviolet difference and circular dichroism techniques at 25 C. Binding isotherms at pH 5·0, 7·0 and 9·0 show cooperative binding at all concentrations of detergents and the number of avai...
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Alphaviruses are arthropod-borne viruses with wide species ranges and diverse tissue tropisms. The cell surface receptors which allow infection of so many different species and cell types are still incompletely characterized. We show here that the widely expressed glycosaminoglycan heparan sulfate can participate in the binding of Sindbis virus to cells. Enzymatic removal of heparan sulfate or ...
متن کامل6xHis promotes binding of a recombinant protein to heparan sulfate.
Commercially available systems provide a variety of strategies for the expression and purification of recombinant proteins. One popular method is the polyhistidine fusion tag, which usually consists of six consecutive histidines (6×His) added to the amino or carboxyl terminus of a protein (7). The high-affinity binding of 6×His to metalchelate resins allows easy purification of 6×His-tagged pro...
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ژورنال
عنوان ژورنال: Bulletin of the Chemical Society of Japan
سال: 1984
ISSN: 0009-2673,1348-0634
DOI: 10.1246/bcsj.57.377